fluorescent microscopy Search Results


membrane tension probe flipper tr  (Cytoskeleton Inc)
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Cytoskeleton Inc membrane tension probe flipper tr
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
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nile red  (Chem Impex International)
95
Chem Impex International nile red
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
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cellsens software  (Evident Corporation)
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Evident Corporation cellsens software
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
Cellsens Software, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunofluorescence microscope  (Evident Corporation)
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Evident Corporation immunofluorescence microscope
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
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er flipper tr probe  (Cytoskeleton Inc)
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Cytoskeleton Inc er flipper tr probe
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
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laser scanning confocal microscopy  (Evident Corporation)
99
Evident Corporation laser scanning confocal microscopy
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
Laser Scanning Confocal Microscopy, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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diode fluorescence microscopy  (Carl Zeiss)
95
Carl Zeiss diode fluorescence microscopy
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
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vs200r esearch slide scanner  (Evident Corporation)
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Evident Corporation vs200r esearch slide scanner
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
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laser confocal scanning fluorescence microscopy  (Evident Corporation)
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Evident Corporation laser confocal scanning fluorescence microscopy
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
Laser Confocal Scanning Fluorescence Microscopy, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lyso flipper tr  (Cytoskeleton Inc)
93
Cytoskeleton Inc lyso flipper tr
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
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confocal microscope  (Evident Corporation)
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Evident Corporation confocal microscope
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
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fluorescence microscope  (Evident Corporation)
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Evident Corporation fluorescence microscope
Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime <t>of</t> <t>Flipper‐TR</t> ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.
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Image Search Results


Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime of Flipper‐TR ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.

Journal: Advanced Science

Article Title: Piezo1 Upregulation in Monocyte‐Derived Macrophages Impairs Post‐Myocardial Infarction Cardiac Repair via Defective Efferocytosis and Enhanced Ferroptosis

doi: 10.1002/advs.202510991

Figure Lengend Snippet: Upregulation of Piezo1 in monocyte‐derived macrophages contributed to increased Piezo1 expression in cardiac macrophages following MI. A) Representative fluorescence lifetime images of freshly isolated bone marrow cells and cultured BMDMs from sham and MI‐operated mice. B,C) The mean lifetime of Flipper‐TR ( n = 6 mice per group, 4 measurements per animal). D) Quantitative PCR analysis of Piezo1 and Piezo2 expression in BMDMs ( n = 5 mice per group). E) Representative Western blots and quantification of Piezo1 protein levels in BMDMs ( n = 5 mice per group). F) Expression of Piezo1 mRNA in blood mononuclear cells ( n = 8 mice per group). G) Piezo1 mRNA expression in whole blood from patients with acute MI and control patients ( n = 10 patients per group). H) Schematic diagram showing the experimental design. I–N) Representative fluorescence‐activated cell sorting (FACS) plot of the GFP + and F4/80 + subsets and statistics of their frequencies in BMDMs I–K) and blood mononuclear cells L–N) from Piezo1 GFP mice ( n = 6 mice per group). O) Schematic diagram showing the strategy for the bone marrow transplantation (BMT) experiment. P,Q) Representative images and statistics of GFP + F4/80 + cells in heart tissues from mice that underwent the Sham or MI procedure after BMT ( n = 5 mice per group). The data in (F,G,J,K,M and N) were analyzed using unpaired Student's t test. Other variables were analyzed using one‐way ANOVA, followed by the Bonferroni post hoc correction.

Article Snippet: Primary bone marrow cells and BMDMs isolated from mice subjected to the MI or sham procedure were incubated with 1 μ m of the membrane tension probe Flipper‐TR (CY‐SC020, Cytoskeleton) for 30 min at 37 °C, as described previously.

Techniques: Derivative Assay, Expressing, Fluorescence, Isolation, Cell Culture, Real-time Polymerase Chain Reaction, Western Blot, Control, FACS, Transplantation Assay